Comparative analysis of the influence of various factors in different
combinations on the ionic homeostasis of a cell.
The ionic homeostasis is one of the most sensitive tests for the evaluation of functional state of a cell.
The agents, which promote elevation of ionic homeostasis, as well as those, which suppress it, are well
known. The goal of the present work was tracing the possibilities for altering ionic homeostasis into
one or another direction, as a result of combined influence of some agents: ionizing radiation at a dose of
0.1 Gy, EMF with frequency of 45 Hz, 2.0 mT, in one case, and addition to this combination of the
homeopathic remedy--stimulated phosphoric acid, at different dilutions. The following data have been
obtained: combination of ionizing radiation of 0.1 Gy increased effect against the effect of its
constituents, while addition of phosphoric acid at dilution of 10(-14) increased effect even further. In
the other series of experiments, combination of ionizing radiation of 0.5 Gy and EMF was used in the
same conditions; as a result, the sum effect decreased against its constituents, while addition of highly
diluted (10(-200)) phosphoric acid practically arrested this effect. It is concluded that effect of a
combined influence depends on a dose of the constituents.
Influence of some homeopathic preparations on tumor cells.
The goal of the study was the assessment of action of homeopathic preparations on ionic homeostasis
in the cells of Ehrlich carcinoma (CEC). Continuous recording of Na(+), K(+), and Ca(2+) with
selective electrodes in the Ringer solution was used as a main method. Activity of the enzymes
controlling transmembrane transport of ions was monitored as well. It was shown that homeopathic
preparation - stimulated phosphoric acid (PA), at low dilutions (10(-14) and 10(-42)), stimulates ionic
transport and Na,K-ATPase in CEC. Non-stimulated PA, at dilutions of 10(-200) and 10(-400),
contrariwise, hampers these indices. It is concluded that structuring of preparation increases with
increased number of dilutions and, respectively "concentration of information field" increases as well.
Transfer of information from initial substance occurs probably in a course of dilution. Such is the
concept of some of the homeopathics.
Influences of some homeopathic preparations on ionic homeostasis at different
Considerable number of the investigations are dedicated to the study of the influences of the various
agents on ionic homeostasis of the cell. However, there are actually no works related to the impact on
these indices of homeopathic preparations (HP). In the present work influence of HP - stimulated
phosphoric acid (PA), at low dilutions (10(-14) and 10(-42)) and non-stimulated PA, at high dilutions
(10(-200) and 10(-400)), on transmembrane transport of Na+, K+, Ca2+, and enzymes - Na,K-ATPase
and Ca-ATPase - was investigated. Experiments were carried out by means of ion-selective electrodes,
in order to measure concentration of ions in the Ringer solution. Results have shown that HP at low
dilution always stimulated observed indices, while HP at high dilution - suppressed these indices.
Therefore, driving force of HP increases with dilution, because number of hydrate complexes increases
and so does velocity of information transmission, which is responsible for different effects.
Influence of electromagnetic radiation of different ranges on the
transmembrane transport of Na+, K+, and Ca2+ ions in normal and tumor cells.
The problem of planetary pollution is one of the most pressing ones. In the present work it was
determined to assess functional state of a cell according to criterion of transport of Na(+), K(+), and Ca
(2+) ions subjected to irradiation with electromagnetic fields (EMF) of different frequency ranges.
Recording of the ions' concentration was made with the ion-selective electrodes immersed into the
Ringer solution. The ion transport was studied in the fibroblasts of the mice. Influences of the EMF in
the range of 20 to 100 Hz and different inductances were assessed. It was found that the frequencies
below 45 Hz and above 60 Hz, at inductance of 2,0 mT induced stimulation of transmembrane
transport of Na(+), K(+), and Ca(2+) ions. According to some electrophysiologists, this effect must be
due to the fact that the frequency below 45 Hz generates potentials too slowly. Therefore an
adaptation to this process does occur, while at the higher frequencies velocity of stimulation is so high
that the cellular membrane cannot react and, as a result, the effect similar to the first variant is observed.
Influence of some low doses of ionizing radiation on the transmembrane
transport of sodium, potassium and calcium ions in normal and tumor cells in
Problem of treatment of various malignant tumors is one of the most acute ones in contemporary
medicine. In evaluation of treatment efficiency it is important to implement the sensitive tests, which
allow precise determination of treatment agent, specifically that of ionizing irradiation. One of such
sensitive indices is the rate of transmembrane transport of Na(+), K(+), and Ca(2+), as well as enzymes
controlling this transport. In the present work characteristics of transport of these ions and enzymes
were assessed during low dose (0,05 and 0,1 Gy) irradiation, which attracts increased attention
nowadays. Recording of the ions' concentration was performed with the ion-selective electrodes. The
cells of Erlich carcinoma were studied. It was found that irradiation with doses of 0,05 and 0,1 Gy,
unlike dose of 0,5 Gy, exerted a stimulating effect on the ion transport. The same is true in regard of
enzymes, which control transport of ions, especially those involved in Ca(2+) transport. The
mechanism of this effect is unclear as yet.